Description

In this experiment, transformants acquire green (GFP) or blue (BFP) fluorescent protein genes contained in two different plasmids. The gfp gene, was originally isolated and cloned from the jellyfish Aequorea victoria, and was mutated to produce the blue protein. In this experiment, transformed cells are selected based on antibiotic resistance. The colonies which express gfp or bfp protein are visibly green and pale blue, and will fluoresce when exposed to long wave UV light commonly known as black light. Shipped ambient. Some components require -20 C storage. A downloadable PDF containing experiment components, requirements, background, procedures and MSDS is available.